ABSTRACT
OBJECTIVE: The aim of this study was to identify apoptosis-related genes of ovarian cancer cell lines following cisplatin treatment. METHODS: We used IC50 values and fluorescence-activated cell sorting analysis to compare cell death in 2 ovarian cancer cell lines, namely, SKOV-3 and OVCAR-3, upon treatment with cisplatin. Moreover, the change in transcriptional levels of apoptosis-associated genes was measured with a dendron-modified DNA microarray. RESULTS: The protein levels for the up-regulated genes in each cell line were validated to identify the molecules that may determine the cellular behavior of cisplatin resistance. Eight genes were over-expressed in the 2 cell lines. The cisplatin-induced up-regulation of DAD1 in transcriptional and protein levels contributed to the cisplatin resistance of OVCAR-3, and the up-regulation of FASTK and TNFRSF11A in SKOV-3 resulted in its higher sensitivity to cisplatin than that of OVCAR-3. CONCLUSION: In the present study, we have identified a set of genes responsible for apoptosis following cisplatin treatment in ovarian cancer cell lines. These genes may give information about the understanding of cisplatin-induced apoptosis in ovarian cancer.
Subject(s)
Apoptosis , Cell Death , Cell Line , Cisplatin , DNA , Flow Cytometry , Inhibitory Concentration 50 , Ovarian Neoplasms , RNA, Messenger , Up-RegulationABSTRACT
The dorsal augmentation is the most common rhinoplastic procedure performed by cosmetic surgeons. To date, however, there are few published cases of rhinoplasty with Gore-Tex in Korea. The present work reports the results of retrospective analysis on photographic reviews and interviews obtained from patients, who have undergone rhinoplastic surgery with Gore-Tex for the past two years in my clinic. A total of 146 patients (23 males and 123 females) with age of 15 to 62 years were monitored for 2 to 24 months after surgery from May 2001 to April 2003. Clinical data was compiled through the in- depth comparison of preoperative and postoperative chart assessment. Both the degree of satisfaction and complaints about foreign body sensations were evaluated based on interviews with patients and measured quantitatively by Leichert scale. The results show that a 75 out of 164 patients were satisfied with surgery outcomes and average points were 3.24(scoring 1 to 5 according to the satisfaction rate). About foreign body sensation, 15 out of 137 patients complained of significant foreign body sensation and average points were 2.12(scoring 1 to 5 according to severity of foreign body sensation). Clinically, the Gore- Tex implant in the cases reported in this study were found to have stability and tissue tolerance.
Subject(s)
Humans , Male , Foreign Bodies , Korea , Polytetrafluoroethylene , Retrospective Studies , Rhinoplasty , SensationABSTRACT
Composite graft and local flaps are good modality for the correction of deformatic scars in facial area but there are several limitations in size, shape and graft bed condition. Hyperbaric oxygen therapy is a good adjunctive therapy for the successful take of the composite graft and local flaps by providing high arterial oxygen pressure and hyperoxic state promoting angiogenesis and collagen synthesis. However, it has some substantial risks of systemic oxygen toxicity to central nervous system, lung and eyes, and needs hyperbaric chamber system and high cost. We designed a modified technique for administrating hyperbaric oxygen topically with the use of simple materials and wall oxygen for the composite graft and local flap wound. 8 patients who had undergone composite graft and local flap on facial scar area were treated with topical hyperbaric therapy for average 6 days. Grafts were relatively large in size and all of the graft bed was scar tissue due to previous operation, trauma and burn injury. All cases showed successful result and there was no complication reported. The potential advantage of this method includes fairly low expense, no need for specialized equipment, simplicity of the application and lack of systemic complication. We conclude that topical hyperbaric oxygen therapy was a simple, cost-effective and safe method for the adjunctive treatment to the composite graft and local flaps.
Subject(s)
Humans , Burns , Central Nervous System , Cicatrix , Collagen , Hyperbaric Oxygenation , Lung , Oxygen , Transplants , Wounds and InjuriesABSTRACT
Four consecutive patients underwent composite resection of tonsillar cancer and reconstruction with a microvascular radial forearm free flap. Three patients had postoperative assessments of speech at six to nine months respectively. One patient could not attend for the postoperative assessment. The speech assessment was examined by same speech therapist. All the patients had an good postoperative speech assessment. Compared to conventional local or regional myocutaneous flaps, the thin forearm free flaps offers better postoperative articulatory recovery.
Subject(s)
Humans , Forearm , Free Tissue Flaps , Myocutaneous Flap , Tonsillar NeoplasmsABSTRACT
Microsurgical techniques have developed numerous territories suitable for free tissue transfer. However, there are so many flaps in plastic and reconstructive surgical field, so no one dare to say which flap is the best. The radiovolar area of the forearm constitutes a versatile source for composite tissue free flap reconstruction for many areas of the body. The skin is thin and relatively hairless, and the vascular pedicle is long and large caliber The flap can be harvested conjunction with tendons and bone. The skin can be reliably reinnervated. We have used 32 radial forearm free flaps to reconstruct defect in various areas of the body resulting from tumor ablation, postburn scar contracture and penile amputation injury. We had two flap failures, one developed necrosis from venous thrombus formation, the other from the infection due to sepsis. Thirty of the 32 flaps survived completely. Overall survival rate was 94%. The donor sites have been relatively inconspicuous.